Organised by the ISO 9001:2015 Certified Exeter Centre for Cytomics (EXCC), University of Exeter
In December 2025, FAILSAFE ran 2 fully funded courses in Flow Cytometry. Both courses provided a comprehensive 3-day online course provided an in-depth introduction to flow cytometry, with a strong focus on practical data analysis and advanced applications. Participants engaged with live expert-led lectures, interactive software tutorials, and remote demonstrations of EXCC’s state-of-the-art platforms. The course featured exclusive access to workflows using spectral cytometry, imaging cytometry, cell sorting, tissue cytometry and multiplex analysis.
What the delegates learnt:
Principles of Flow Cytometry: Gained a solid theoretical foundation in fluidics, optics, fluorescence, and data acquisition. Understand how flow cytometry works and how it applies across biological disciplines.
Spectral Flow Cytometry: Explored the advantages of spectral systems through real-world demonstrations and case studies. Learned about unmixing, autofluorescence correction, and panel design using instruments such as Cytek Aurora, Bigfoot, and FACSymphony S6.
Cell Sorting: Understand setup procedures, sort logic, and biosafety considerations for advanced sorters including FACSAria Fusion, Bigfoot Spectral Sorter, and FACSymphony S6.
Tissue Cytometry: Learned how to apply MACSima Tissue Cytometry for multiplexed spatial proteomics and transcriptomics using imaging-based workflows.
Basic Flow Data Analysis: Gained confidence in FCS data structure, quality control, gating strategies, statistics, and batch processing using FlowJo and FCS Express.
Advanced High-Dimensional Data Analysis: Explored dimensionality reduction and clustering tools such as t-SNE, UMAP, FlowSOM, and Phenograph, supported by real-world immunophenotyping case studies.
Built competency in open-source tools including cytometry analysis in R and Python
Who was this course for?
This course was ideal for researchers, technicians, and students aiming to deepen their understanding of both conventional and high-parameter flow cytometry. Whether they were new to cytometry or looking to expand thier capabilities into spectral platforms, single-cell analysis, or machine learning-supported workflows, this course provided the practical and analytical tools to advance your work.
Course Dates
This course was be offered twice to accommodate global participants across different time zones and ran on the following dates:
• First session: 2–4 December 2025, starting at 1:00 PM UK time (6 hours per day)
• Second session: 9–11 December 2025, starting at 7:00 AM UK time (6 hours per day)
To explore all of our upcoming FAILSAFE courses and learn how to participate, click here.
Don’t miss your chance to join these transformative training opportunities—membership in the CMM-FAILSAFE Network is required. If you’re not already a member, joining is completely free and simple. Just click here and complete the short registration form to get started today.
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The application period for the Flow Cytometry Course 2025 has now closed. We appreciate everyone who applied. All successful candidates have been notified, and we look forward to welcoming you to the course.
